Comparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR in Uganda with Quantitative Real Time RT-PCR on Field Samples

dc.contributor.authorMukasa, Hussein Kafeero
dc.date.accessioned2018-02-21T15:34:16Z
dc.date.available2018-02-21T15:34:16Z
dc.date.issued2016
dc.description.abstractFoot-and-mouth disease (FMD) is a viral disease of Ungulates; both Artiodactyla and Perissodactyla. The mortality rates are low in adult animals but it affects milk yield and international trade. In endemic countries, diagnosis can be based on clinical signs. But these are shared by other vesicular diseases, so a laboratory is needed to confirm the disease. In Uganda the commonly used assays for the laboratory diagnosis of FMD are NSP ELISA and RT-PCR. Serology using ELISA techniques may fail to distinguish between vaccinated and new infection so compromising its sensitivity. The gel passed PCR is involves a lot of advance sample treatment increasing errors due to carry over which also compromises its sensitivity. This work reports comparative the detection of foot-and-mouth virus by NSP ELISA and RTPCR with real time PCR which was taken as the gold standard. The assays were compared in terms of sensitivity, specificity and disease prevalence and likelihood ratios. A total of 176 cattle were used from which samples that included epithelial tissues (17.05%) and oral swabs (84.09%) were collected from outbreak cases in Eastern Districts of Mbale and Budaka.en_US
dc.identifier.citationMukasa, H.K ... et al (2016) Comparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR in Uganda with Quantitative Real Time RT-PCR on Field Samples. Global Journal of Medical Research. Vol.16 (2)en_US
dc.identifier.issn0975-5888
dc.identifier.issn2249-4618
dc.identifier.urihttp://hdl.handle.net/20.500.12309/495
dc.language.isoenen_US
dc.publisherGlobal Journal of Medical Researchen_US
dc.subjectSP-ELISAen_US
dc.subjectRT-PCRen_US
dc.subjectSensitivityen_US
dc.subjectFoot-and-Mouth Diseaseen_US
dc.subjectUgandaen_US
dc.subjectReal Time PCRen_US
dc.subjectFocal screeningen_US
dc.titleComparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR in Uganda with Quantitative Real Time RT-PCR on Field Samplesen_US
dc.typeArticleen_US

Files

Original bundle

Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
1123-1-.pdf
Size:
685.42 KB
Format:
Adobe Portable Document Format
Description:

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:

Collections