Browsing by Author "Awotunde, O.S"

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Antioxidant activities of Hydro-ethanol and Saponin extracts of Terminalia schimeperiana root
(Der Pharmacia Lettre, 2017) Awotunde, O.S; Dhanabal, S.P; Rajeshkumar, Raman; Chasitainya, MVNL
In this present paper we have investigated the Antioxidant activity of Hydro-ethanol and Saponin fractions of Terminalia schimperiana root for its free radical scavenging activity by adopting ABTS and DPPH in vitro methods. The extracts were investigated for the antioxidant activity using 2, 2 - diphenyl, 1- picryl hydrazyl (DPPH) and 2, 2 azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging activity, reducing capacity and competition with DMSO. The result suggested that the polar Hydro-ethanol fraction was found to have potent DPPH antioxidant activity with IC50 value of 19.36± 0.436 μg/ml and ABTS scavenging activity with IC50 value of 0.9420± 0.011 μg/ml, while the Saponin fraction has moderate DPPH scavenging activity with IC50 of 59.33± 0.417 μg/ml and moderate ABTS scavenging activity with IC50 value of 2.273± 0.036 μg/ml (Rutin DPPH IC50 value= 14.5±0.29 μg/ml, Rutin ABTS IC5O value= 0.2976± 0.012 μg/ml, Ascorbic acid ABTS IC50 value= 2.62± 0.20, Ascorbic acid DPPH IC50 value=9.51± 0.22).
Evaluation of Proximate Composition and Phytochemical analysis of Terminalia Schimperiana Root.
(Academia, 2017) Awotunde, O.S; Adewoye, S.O; Hawumba, J; Nuwamanya, E
The Proximate composition and phytochemical characteristics were determined for Terminalia schimperiana root. The root was collected and extracted with water and the phytochemical screening was carried out using the standard procedures. Phytochemical screening of the aqueous extract of Terminalia schimperiana root revealed the presence of alkaloids, saponins, Phenolics, anthocyanin and Tannins. The Proximate analysis results obtained showed the amount of crude protein, crude fat and total carbohydrates to be 6.31±0.75, 4.64±0.82 and 17.79 ±0.53 respectively of the sample while the percentage of crude fiber, ash and moisture content in the root sample were 32.72 ±0.59, 0.35±0.12 and 10.35±0.18 respectively. The present study revealed that the plant contain many nutrients and phytochemicals that may be responsible for some medicinal and aphrodisiac properties.
Histopathological and Organ-Body Weight Ratio Alterations Induced by Oral Administration of AQUEOUS Extract of Terminaliaschmiperiana Root in Some Selected Organs in Male WISTAR Rats
(International Journal of Advanced Research in Science, Engineering and Technology, 2017) Awotunde, O.S; Adewoye, S.O; Hawumba, J.; Alinaitwe, Lordrick
The toxic potential of the crude aqueous extract was investigated by evaluating the body-weight ratioand histologic changes of the liver, kidney and testes after oral administration of the extractto rats at various doses (1000, 2000, 3000mg/kg) daily for21 days. On the first, seventh and twenty first day,the rats werehumanely sacrificed under chloroform anesthesiaand the selected organs excised. There was no significant difference (P<0.05) in the kidney-body weight ratio when compared with the control group, while the administration at all the doses resulted in significant (P<0.05) reduction in liver-body weight ratio.An increase in testes-body weight ratio was also observed at the dose of 2000mg/kg. Histopathologicexamination of the liver, kidney and testes after 21 days of administration of the extract revealed mild changes in the architecture of the tissues.The male wistar rats did not show any treatment-related defects nor overt clinical signs of toxicity neither did the extract produce any sedative effect.
Sexual behavior effect of orally administered crude aqueous extract of Terminalia schimperiana Root in male Wistar rat
(Plant Journal, 2016) Awotunde, O.S
The Proximate composition, phytochemical composition and the effect of oral administration of aqueous extract of Terminalia schimperiana root on male wistar rat sexual behavior were determined. The root was collected and extracted with water, the phytochemical screening was carried out using the standard procedures and revealed the presence of alkaloids, saponins, Phenolics, anthocyanin and Tannins. The Proximate analysis results obtained showed the amount of crude protein, crude fat and total carbohydrates to be 6.31±0.75, 4.64±0.82 and 17.79 ±0.53 respectively of the sample while the percentage of crude fiber, ash and moisture content in the root sample were 32.72 ±0.59, 0.35±0.12 and 10.35±0.18 respectively. Aqueous extract of Terminalia schimperiana root was orally administered to sexually active rats in a daily dose of 1000, 2000 and 3000 mg/Kg body weight for 5 days, the sexual behavior was monitored and testosterone serum level was measured on Terminalia schimperiana treated rats, control rats “that received vehicle” and the reference group “ that received Powmax”. Oral administration of Terminalia schimperiana significantly increased the mount frequency, intromission frequency and prolonged the ejaculatory latency. In addition, a significant increase in the copulatory efficacy was reported for the test groups and testosterone serum level was significantly increased in Terminalia schimperiana treated rats compared to controls and reference. The present study revealed that continuous administration of Terminalia schimperiana aqueous extract for a period of 5 days improves the sexual motivation and performance in adult male rats, an effect that may be linked to increased testosterone level, the nutrient composition and the phytochemicals.